Neuro-Electronics Research Flanders

Functional ultrasound imaging and 2P imaging to study the neurovascular unit

20 November 2017

Alan Urban, Clément Brunner and Gabriel Montaldo (Urban lab) teamed up with researchers in Tel Aviv and Milan to write an extensive review on the advances in two complementary techniques: multi-photon laser scanning microscopy (MPLSM) and functional ultrasound imaging (fUSi). 

MPLSM has become the gold standard for in vivo imaging of cellular dynamics and morphology, together with cerebral blood flow. fUSi is an innovative imaging modality based on Doppler ultrasound, capable of recording vascular brain activity over tens of cubic millimeters at unprecedented resolution. By merging these two technologies, we can study the neurovascular interface in even greater detail.

The combination of technologies would not only advance the study of brain function, ranging from a single cell to large circuits, but also help to evaluate the effects of novel pharmaceutical compounds. It could become an efficient preclinical tool for middle-throughput in vivo drug screening.

Alan Urban, Lior Golghere, Clément Brunner, Amos Gdalyahu, Hagai Har-Gil, David Kain, Gabriel Montaldo, Laura Sironi & Pablo Blinder. Understanding the neurovascular unit at multiple scales: Advantages and limitations of multi-photon and functional ultrasound imaging. Advanced Drug Delivery Reviews, 15 September 2017, Pages 73-100 (2017) doi.org/10.1016/j.addr.2017.07.018